I have isolated bacillus subtilis from different sources of soil, is there any biochemical method for identification of bacillus subtilis species? Please inform me how to confirm the bacteria biochemically.
A very simple and quick method is the use of chromogenic agars (see www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Fluka/Datasheet/92325dat.pdf). Bacillus subtilis should yield green colonies and can be differentiated from other bacilli. However, this is probably only a quick “pre-test” and should be confirmed by other tests. If you are looking for biochemical identification you might use e.g., API or BIOLOG.
Dear Helumut Brandi,
Thank you very much for your time i will go through the catalog n follow the procedure , i will let you know how the result progress.
Thank you
Dear Pavan,
You can use the Attana technology. Depending on what you are after you can also get more than just identification.
Please contact me if you want more information.
Teodor
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