Pavan Kumar

4 Questions 11 Answers 0 Followers

Questions related from Pavan Kumar

What are the Manual Protocol for isolation of DNA from Soil ?

Dear Friends, Is there any protocol to isolate good quality DNA manually from soil? we are targeting for both bacteria and fungi, so is there any manual methods for isolation, we don't need kit...

08 August 2017 1,602 6 View

Is there Manual/ kit protocol for isolation of total RNA from Pomegranate leaves?

Dear friends, We are trying to isolate total RNA from pomegranate leaves, we tried using several kits but purity was the problem and it requires an additional manual purification for better...

07 July 2016 5,568 4 View

Is there a biochemical method for identification of bacillus subtilis?

I have isolated bacillus subtilis from different sources of soil, is there any biochemical method for identification of bacillus subtilis species? Please inform me how to confirm the bacteria...

04 April 2014 6,924 3 View

Where may I have gone wrong in the amplification of RAPD marker in PCR?

We isolated DNA from plant sample when I tried to amplify with RAPD Markers I am not getting any band in the gel (ref. picture), in 20µl of pcr mixture I took 50ng of DNA and 20pico mol conc. of...

02 February 2014 7,550 11 View