Dear researcher all,
I'm looking for best protocol for primary culture of colonic or cecum intestinal epithelium cells (IEC) from mice. The strain of mice is C57BL/6. Does anyone know how to culture IEC from mice and successful experience?
Best regards,
Dear Yugo Karatsu,
I would suggest for you to check out the procedures in the following article:
Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis.
https://www.nature.com/articles/mi201398
Here, researchers adapted a previously developed method for the culture of intestinal epithelial spheroids to establish primary epithelial cell monolayers from the colon of multiple genetic mouse strains, so that may work out for your purposes.
I also found this other article:
Epigenetic Control of the Host Gene by Commensal Bacteria in Large Intestinal Epithelial Cells
Article Epigenetic Control of the Host Gene by Commensal Bacteria in...
The preparation of IECs is described as part of the experimental procedures, in which they prepare single cell suspensions of IECs from small and large intestines (color and rectum, however, not including the cecum).
I hope you find the procedures in these articles useful.
Good luck and best regards.
Dear Fernando Quinto-Ureña,
Thank you for referring me to the two papers. The first one is that they have been able to culture cells derived from pIgR knockout mice without any problems, and I think we can do even better with WT. Also, I'm going to check second one.
We will try even harder to make the experiment work.
Best regards,
Hi,
Let me know if your experiment worked and if possible please send me the detailed protocol. I am trying to set up a primary culture of intestinal epithelial cells from mice.