I am trying to culture Primary mouse Intestinal epithelial cells from Mice. I am not getting any viable cells ?

The Protocol I am following is as follows : 1. Isolate the Proximal and cecal part of the colon in ice cold DPBS. 2. Flush the colon using cold DPBS to remove the stool. 3. Put the colon on a wet...

12 May 2022 2,850 0 View