How can I differentiate between the phenotypes of resident macrophage and elicited macrophages
I am looking for professional software to draw the physical structure of nanoparticles or vesicles, not the chemical structures for which we use ChemDraw, but the actual physical structures,...
23 June 2024 1,292 3 View
I have done caspase-glo 3/7 assay which measures caspase activity after cleavage and generation of luminescent signal produced by luciferase. However,after I got the results, I am not sure how to...
02 June 2024 7,833 2 View
Hi, I've observed a new biological activity in certain cells, likely induced by an enzyme. How can I determine the gene or genes responsible for expressing this unknown enzyme? Thanks in advance
31 May 2024 9,111 3 View
I have many skills like data analysis with R programming or Spss , scientific writing and paper publishing
23 May 2024 1,683 3 View
I have cells that produce GFP as a control, and I intend to perform the MTT and Caspase 3/7 Glo assays. The emission wavelength of GFP is 508 nm, and its absorption is 475 nm. In addition, I will...
16 April 2024 3,537 2 View
The positive curves for E coli different strains from mucual stool sample.
29 March 2024 5,907 0 View
methylation of spata 6 gene and its role on male infertility of rat
08 March 2024 9,667 0 View
how to prepare concentration MPs for field scale ( phytoremediation of floating plants ( water lily, water mimosa, water hyacinth )?
04 March 2024 1,822 0 View
I am a researcher who decided to buy labtop suitable for my work as a simulation and data analysis work Please suggest one
23 February 2024 3,635 2 View
Note: Serum isolated extracellular vesicles using immunoprecipitation (magnetic beads)
20 February 2024 4,894 1 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
01 August 2024 476 2 View
I have a question about human macrophages cell surface markers.I want to stain the unpolarized human macrophages for flow cytometric sorting without permeabilizing them. It is because I need to...
29 July 2024 236 2 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...
15 July 2024 5,153 2 View
Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...
15 July 2024 4,615 1 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...
03 July 2024 3,884 5 View
Hi everyone, I am trying to induce foam cells in macrophage differentiated from PBMC. Which type of staining do you prefer to use? Nile Red or Oil RED O ? What concentration and timing of ox-LDL...
01 July 2024 8,286 0 View