Which is the easiest and most reliable way to check the viability of lactobacillus apart from the plating techniques?
Hey Divya
Yes the dead/alive staining procedure is a reliable method to determine the survivability of Lactobacillus species. The method can discriminate as little as 1–10% live or dead cells in a mixed population.
The LIVE/DEAD® BacLight™ Bacterial Viability Kits (Invitrogen) employ two nucleic acid stains—green-fluorescent SYTO® 9 stain and red-fluorescent propidium iodide stain. These stains differ in their ability to penetrate healthy bacterial cells. When used alone, SYTO® 9 stain labels both live and dead bacteria. In contrast, propidium iodide penetrates only bacteria with damaged membranes, reducing SYTO® 9 fluorescence when both dyes are present. Thus, live bacteria with intact membranes fluoresce green, while dead bacteria with damaged membranes fluoresce red.
The staining procedure is simple and the visualization of the stained bacteria by either fluorescence microscopy or flow cytometry is easy to conduct.
Regards
Deon
But I want to know about FDA staining and visualizing under optical microscopy using counting chamber slide sothat we can calculate live and dead cell percentage in the sample?
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