How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

What keeps journals from switching to a "publish, then review" model of publishing?

A few scientific journals have started implementing a "publish, then review" model of publishing. It is explained well in this article: Implementing a "publish, then review" model of...

13 January 2021 5,449 2 View

Is there any project for a new translation of Freud's 1895 Entwurf einer Psychologie into English?

I'm far from being a Freud expert, but, my feeling is the 1950 translation by J. Strachey is in many ways outdated, when not misleading (starting form the very title of the essay, the awful choice...

10 December 2020 7,005 2 View

What is the main difference of diluting HCL in methanol as opposed to water?

I am hoping to determine the mass per unit area (as per DIN 50938) of a black oxide coating. I use water to achieve the required 1mol/l hydrochloric acidic solution, but a fellow lab had used...

06 December 2020 4,806 1 View

Linear Mixed Models for repeated measures - how can I restructure my data set when some variables are only collected at baseline?

Hello, I plan to perform a linear mixed model analysis to look at change in cognitive function over 5 follow up waves. I am interested in looking at how this change is influenced by dietary and...

27 November 2020 5,087 3 View

Mendeley profiles will be removed in December. What will be of the reader counts from Mendeley as early impact predictors?

Elsevier announced that Mendeley profiles will be discontinued from December. There is a study (Mendeley readership counts: An investigation of temporal and... ) that confirms the value of...

01 November 2020 4,832 4 View

Assessing MCMC convergence by matrix-parameter?

Hello everyone, I need to assess whether a MCMC chain (implemented in BayPass 2.2) has reached convergence and, as far as I know, in order to check it, I must look at the posterior distribution of...

27 October 2020 6,170 3 View

Searching for reliable antibodies for the adrenergic receptors alpha1D and alpha2A for western blotting?

Dear all, I am looking for reliable antibodies for the adrenergic receptors alpha1D and alpha2A, to use in Western Blotting on human skeletal muscle tissue. Anyone with experience with good...

25 October 2020 9,421 2 View

What is the best way to start and develop a scientific collaboration?

What advice would you give to a young researcher who has to develop his/her ability to produce collaborative scientific work?

22 October 2020 5,934 8 View

Temporal noise analysis CMOS APS?

I was reading the paper "Analysis of Temporal Noise in CMOS Photodiode Active Pixel Sensor" but I am a bit puzzled about noise calculation during integration. In particular, can someone explain...

15 October 2020 9,597 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View