For the following four inbred lines; W, X, Y and Z of a species the following Ct values were obtained:
Ct gene: 21, 22, 23, 22
Ct ref: 18, 20, 19, 17 respectively.
In this case, I would like to consider 'line W' as a calibrator (control).
Can you please show me a sample calculation for these data?
18-21=-3, in excel use formula =power(2,-3), then set it 1 by dividing the value of excel,
for DELT CT VALUE . DELTA CT OF TARGET GENE- DELTA CT OF CONTROL
i.e 21-18= 3
22-20= 2
23-19=4 all these are delt ct value. put double delta ct value 0 for wt control and put 1 for 2 to power minus double delta ct value.
now calcultae double delta ct value for other samples by using formula delta ct value of target- delta ct value of control or calibrator.i.e 2-3= -1 and put this value in 2 to the power double delta ct value. now ans will be 2. for next 2-4=-2 now 2 to the power minus double delta ct value is= 4. if u want
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