I need to insert a gen systemically, but with the skeletal muscle as specific target. Is the adeno-associated virus type 6 (AAV6) vector a good vehicle? Do you know other options?
In humans and dogs the AAV6 can be blocked by the G3BP, not in mice...Maybe it's better the AAV9.
Take a look at his paper:
Human Galectin 3 Binding Protein Interacts with Recombinant Adeno-Associated Virus Type 6. Denard et al. 2012.
Journal of Virology ; June 2012 Volume 86 Number 12
Specific targeting of muscle after systemic administration will be challenging. Most studies targeting muscle use local administration, such as whole limb perfusion or i.m injections to restrict the transduction to the muscle. Our group has done this using AAV6 in dogs and it works well.
Thank you Isabel. However I have read that AAV9 is specific for heart and liver more than skeletal miscle. Isn´t it?
AAV9 has a good muscle transduction levels in muscles, but it has been proven in mice to have a better tropism to heart muscles.
AAV9 seems to be a wonder vector, working in the brain, muscle, liver, etc.
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