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Questions related from Jonathan D Finn
I am interested in working with the split GFP system (GFP1-10 on on protein, GFP11 strand fused to another). Almost all examples I have seen have fused the GFP11 strand to the N or C terminus....
09 December 2020 3,345 0 View
Does anyone have insight as to whether the spectrum of peptides presented from a protein will be different whether the protein is expressed in the cell (e.g. gene delivery) via direct delivery of...
27 April 2016 5,639 2 View
A lot of people use lambda phage DNA as stuffer DNA for a variety of applications. I was wondering if anyone knows if there would be any expression of the phage genes if the stuffer is used in...
29 November 2015 1,703 3 View
One of the reasons why adenovirus has short term expression is due to the strong immune response that it generates. This anti-vector immunity usually limits the expression to ~1-2 weeks. It is...
02 November 2015 6,542 6 View
Hello, As a researcher working primarily in the AAV field, I am well aware of the issue with neutralizing antibodies against AAV that prevent re-administration of vector. I have never heard...
02 April 2015 7,735 1 View
Hello genome editors, When using the CRISPR-Cas9 system, does anyone know how long the gRNAs are usually expressed for? I know that they are generally expressed from a constitutive pol III...
07 October 2014 6,414 2 View
I know that magnetite nanoparticles are being investigated as contrast agents for MRI scans (for example http://www.sciencedirect.com/science/article/pii/S0272884210000611). Why would the very...
29 July 2014 816 9 View
I know that for some transcription factors, binding to their target sequence can turn genes on or off, but for NFkB, I have only seen examples where it turns gene expression on (for example, after...
02 May 2014 594 3 View
