Yes, bile-stain is only positiv in liver, when there is something like a blockade and reflux of bile. In clinical pathology a positive Hall stain is a sign for e.g. stone or tumour in efferent ways. as described in the protocol  - biliary obstruction.

Thanks Gudrung for your answer, I ve attached some photos from my staining, would appreciate if anyone can help and give me some clarification.

Thanks in advance