18 March 2015 1 996 Report

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More Akshi Anbu's questions See All
What are the various dilemmas or polarities faced by leaders in NGOs?

Non governmental organizations exist with an intent to serve the society for the welfare of the people. However, these NGOs also are faced with several challenges in terms of leadership, HR,...

24 May 2024 5,710 0 View

Is there is any reference books for conjugate gradient method for PDE?

Conjugate gradient method for PDE

13 September 2017 936 3 View

What purpose of NH4F In COAL-LDH Preparation

many  reported literature showed the COAL-LDH with out NH4F  while in some literature NH4F have been used with COAL -LDH preparation . hat is the main purpose to NH4F  In synthesis of COAL -LDH ?

10 May 2017 5,169 2 View

Anyone knows how to dissolve palmitic acid, stearic acid and myristric acid and these FFAs - BSA conjugation protocol ?

How to avoid  precipitation  

14 March 2016 9,131 2 View

How do I conjuagate FFA's (Linoleicacid, Oleic acid, Palmitic acid (sigma)) with FFA free BSA detailed protocol ?

I have seen some protocols are showing sodium palmitate conjugated with BSA but i need FFA's (Linoleicacid, Oleic acid, Palmitic acid (sigma)) with FFA free BSA (sigma) conjugated specific...

20 September 2015 3,766 3 View

Can neutral buffered formalin 10% be prepared in 1X PBS ?

For fixation of cells in 10% formalin with PBS . 1xPBS with 37% formaldehyde  can be used 

21 July 2015 7,894 2 View

Which method is best for preparing FFA BSA conjugate by ethanol or DMSO?

I have searched many articles but i have no idea to decide which is best to carry on am going to coculture adipocytes cell line with macrophages and induced mimic obesity so am using FFA BSA...

10 May 2015 3,411 2 View

Is anyone currently working Ion Torrent Sequencing (ITS)?

Is anyone currently working on fungal sequences generated from an Ion Torrent Sequencing platform and using mothur for analyzing those sequences?

24 November 2014 7,755 1 View

How to control the degree of polymerization when we synthesise triblock polymers and how to purify the material?

I am synthesizing triblock copolymer for anticancer gene delivery. I want to know how can i control the degree of each block to achieve a efficient gene delivery vector.

08 December 2013 4,233 10 View

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Could it be a cell culture contamination?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

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Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

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How to isolate lymphocytes from mouse spleen?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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