Hello forum,
I would like to know if TBA.OH is volatile in mixtures of acetonitrile or methanol. I want to analyze peptides that were previously dissolved in TBA.OH-conatining solution, but dried and re-dissolved in 0.1% FA. Would TBA.OH have been evaporated?
Thank you!
Please look at the following below links which may help you in your analysis:
http://aether.cmi.ua.ac.be/artikels/MB_32466.pdf
https://pubmed.ncbi.nlm.nih.gov/23919350/
https://pubs.acs.org/doi/abs/10.1021/ac401222b
Thanks
I assume you are working in LC/MS even if you didn't make it clear. TBAOH is not volatile and won't disappear upon drying. Being unclear what the original TBAOH concentration was, I suggest to check if the 0.1%FA is sufficient to balance the base. Consider that TBAOH is a strong modifier (ion-pairing agent) and weird things could happen to your chromatography. Another aspect is the base strength which rises on drying, leading to possible degradation reactions on peptides, specially if the mixture is heated.
Good work
Unfortunately, it won't be blown away. It is a common issue to deal with ion suppression effects at MS systems due to sticky nature but also it affects your separation efficiency which represents a stagnant phase at your column when using surfactants at your mobile phases. Bulk quantities at your final injection solvents can cause similar observation but in case of minor concentrations, I guess that it won't be a problem. It is always the best to remove surface-active molecules before MS injections for healthy chromatography and secure ionization since these molecules can interact with your peptides, the column, and also with your consumables resulting in low recoveries.
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