Is Sytox (from Thermo Fisher Scientific) a good choice to dectect NETs in flow cytometry using whole blood?

More Luca Galgano's questions See All

Lymhpoma cell culture issues, help?

Hi! I need help for some issues in growing mantle cell lymphoma cell lines (REC-1, MAVER-1, Granta-519, Mino, Jeko-1). I noticed that it is a little bit hard to maintain a stable cell culture:...

09 February 2021 7,402 11 View

Help to set up an in vitro Bioaccessibility assay?

Hi everyone! i am looking for protocols to evaluate the bioaccessibility of carotenoids in plant matrix. Ideally the best would be a quite easy protocol that could be used massively in the lab,...

02 February 2021 5,516 2 View

How seminal was the influence of Giuseppe Campos Venuti and Federico Oliva in Italy, France, Spain and other countries?

The question is what remains in the urban planning and in our professional and intellectual experiences of Giuseppe Campos Venuti and Federico Oliva? What about discipline now? They reasoned and...

30 October 2020 5,450 4 View

HPLC Alternative for a new project?

dear collegues, i would like to know if there are any high throughput and specific techniques alternative to the HPLC analysis for protein detection and if possible their relative costs. Thank...

04 October 2020 9,928 3 View

Maximum concentration for HPLC???

Dear Collegues, I'm starting a new project and I need to know what is the maximum concentration for a sample that have to be processed by analytical HPLC. Thank you so much for the answers! Luca

01 October 2020 1,654 5 View

How to make ethyl cellulose water soluble?

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20 July 2020 8,592 1 View

Single cell RNA sequencing on immune cells infiltrated in tissues: what is the best way?

I am planning to investigate how immune cells infiltrated in certain tissues may change between different mouse models. If I don't want to focus on a specific immune cell type, is it possible to...

30 June 2020 1,834 3 View

Empatica E4 data processing tools and strategies?

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14 June 2020 6,185 2 View

Binary Classification - Machine Learning - When using which one?

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28 April 2020 4,728 4 View

Looking for (MSc course) syllabus in the field of social innovation / territorial development / urban studies . Can you suggest any material?

Social Innovation courses are often declined in a way that is often attached to social entrepreneurship or design methods (i.e. theory of change). Can you suggest any material on how to draw a...

06 February 2020 4,607 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

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03 March 2021 5,978 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

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02 March 2021 1,999 5 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

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01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What could be some reasons why unstimulated monocytes are getting activated?

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28 February 2021 7,883 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

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25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

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25 February 2021 3,443 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Walther Haenseler

Have not done NET assay in the last 8 years or so, but back then they were microscopy based. Cannot imagine that the exploded cells go easily through the flow cytometer...

Qiong-Yi Hu

It's not specific for Sytox-green to detect NET only. And to detect NETs in blood is also not specific.