I'm trying to get hold on B3Z cells for my assay. Would someone be ready to share these cells?
Thanks for your help
Hello, I am new to designing flow cytometry experiments. I have some cells transfected with EGFP using mRNA and want to quantify its percentage expressed. I also wish to differentiate between...
17 May 2024 8,527 2 View
Hello RG community, I am looking for remote sensing data on cloud cover estimation on a daily basis but during nighttime over Namibia /Southeast Africa. The ideal spatial resolution would be 1km...
31 March 2024 298 2 View
I am currently doing isolation of protein from inclusion bodies. I solubilize the protein with 8M guanidine hydrochloride (Simpson, CSHL, 2010). I this step I saw no pellet anymore in my sample....
17 March 2024 8,573 0 View
How to form the immune complex using human IgG?
16 November 2023 3,811 1 View
I want to study the male reproductive system for two different seasons to obtain histology, histometery, morphology and morphometery. Please guide me how much should be my simple size. What would...
22 May 2023 7,177 5 View
I am working on my dissertation, and I am stuck on the type test I should run on my data. Research Design-I have a control and a treatment group where a vocabulary intervention was given to the...
02 April 2023 1,263 4 View
I am trying to do a shRNA gene silencing for an esophageal cell line. Does anyone have any specific protocol to suggest?
27 March 2023 6,675 3 View
I performed a reaction to modify BSA through esterification, but it didn't seem to work. Most of the papers that I've found that attach azide to BSA seem to use maleimide. Is there a reason why...
09 March 2023 4,971 2 View
policies initiatives during past few years
08 March 2023 5,540 2 View
How do we as scientists interpret data to provide the most explicit explanation for our research? I am aware that we can present the limitations of our study which can defer our...
15 February 2023 2,867 1 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
10 July 2024 4,918 2 View