Dear Agostino,

CD25 is not restricted to T cells, it is expressed by several other cells including B cells, dendritic cells, eosinophils and macrophages. Because of macrophage CD25 expression, increased sCD25 can be seen in diseases involving macrophage activation (J Allergy Clin Immunol. 1994,94:1057, Arthritis Rheum. 2007;56:965).

Yours

Loems

Thanks Loems! Do you know if there's some activated T cell marker that I could find in serum that's specific for those type of cells?

To my knowledge there is no such specific marker. The best approach still is staining of T cells for CD25.

Thanks for your help! Ago

Dear Agostino,

although CD62L (L-selectin) is also expressed on other cells, you might look, whether T cell activation in your model is correlated to increased levels of soluble L-selectin, which might be shed from the T cell surface upon activation. You might combine your approaches with simultaneous measurement of surface L-selectin on T cells and on other cell populations (for example, granulocytes, NK cells, B cells).