I'm wondering if there's a specific serum marker of activated T cells.
Dear Agostino,
CD25 is not restricted to T cells, it is expressed by several other cells including B cells, dendritic cells, eosinophils and macrophages. Because of macrophage CD25 expression, increased sCD25 can be seen in diseases involving macrophage activation (J Allergy Clin Immunol. 1994,94:1057, Arthritis Rheum. 2007;56:965).
Yours
Loems
Thanks Loems! Do you know if there's some activated T cell marker that I could find in serum that's specific for those type of cells?
To my knowledge there is no such specific marker. The best approach still is staining of T cells for CD25.
Dear Agostino,
although CD62L (L-selectin) is also expressed on other cells, you might look, whether T cell activation in your model is correlated to increased levels of soluble L-selectin, which might be shed from the T cell surface upon activation. You might combine your approaches with simultaneous measurement of surface L-selectin on T cells and on other cell populations (for example, granulocytes, NK cells, B cells).
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