Is soil organic carbon content affected by drying it in hot air oven ??

More Manas Ranjan Mohanta's questions See All

As a research we need to publishh our work, most of us spend maximum time in writing a journal, submiting it, but at the end get rejected why?

story of every researcher

31 March 2024 1,732 5 View

XRD peak deviation?

In XRD analysis, when we move to a higher angle, like above 50 degrees, if I have two reference lines of different phases, one matches the peak and the other is towards the right of the peak with...

31 March 2024 1,812 2 View

Will the temperature of PCR lid affect the overnight ligation at 16 degree celsius?

I had set up overnight ligation reaction at 16 degree celsius in a PCR machine but the lid temperature was high around 95 degrees. Will it affect the efficeincy of ligation if done in PCR in this way?

22 January 2024 2,587 2 View

Is it possible for yttrium or yttrium oxide to react at 760 degree Celsius during casting of magnesium, if its added to reinforce the magnesium?

Casting was done under SF6-CO2 and argon gases environment.

02 December 2023 4,920 2 View

Is there any non kit based method to isolate PCR amplified product?

Is there any manual method to precipitate PCR amplified product?

01 December 2023 5,539 4 View

For doing blunt end cloning shall I dephosphorylate the linearized vector before blunting?

To ligate blunt insert into blunt vector does the vector need to be dephosphorylated at the time of ligation? I have digested my vector with restriction enzyme that create sticky ends that...

15 November 2023 448 0 View

Will the gene under EF1a promoter (from mammalian expression vector) be expressed in any bacterial host?

I have inserted a gene in downstream to Ef1a promoter of my mammalian expression vector. Can I check the protein expression using any bacterial expression host? Or, will it only express in any...

06 November 2023 3,410 4 View

What are the odds that, the amplicon generated using primers carrying overhangs will have blunt end and does not carry any overhangs ?

The DNA polymerase fills in the gap due to overhangs (using a pair of primers carrying overhangs) during the 2nd round of replication in PCR. Does those gaps actually get filled or they have...

02 November 2023 6,400 1 View

How can I ligate an insert digested with same restriction enzyme from both end to a vector digested with same enzyme?

I have insert having flanking regions with XbaI site and and a vector digested with XbaI. How can I prevent self ligation in insert as well as vector before ligation?

31 October 2023 8,865 1 View

How can we develop robust & scalable strategies for precise gene editing in pluripotent stem cells, allowing us to correct disease-causing mutation ?

Even though we have advanced so much into using Genome with Technology to cure diseases, still newborn babies are suffering of abnormal genetic mutations. How can we scale these methods to make...

12 June 2023 6,781 0 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Who of all of you will help TPEOM to re-originate the Earths atmosphere to it original 5 atmospheric elements/gases?

Global ‘Weather’ has stopped, as ‘aqua thermionic instituted by the ‘Global thermo atmospherics has instituted global thermodynamics of 120º C + and thus all waters are, or have boiled into a...

06 August 2024 8,716 0 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Andrew Paul McKenzie Pegman

No, carbon will not be degraded at those conditions