We used WB to analysis tumor sample from Hepa1-6 transplanted to C57BL6J mouse flank. In drug treatment group, we observed reduced p62 level and accumulation of LC3B-I.

We assumed that reduced p62 level indicates that autophagy is stimulated. However, accumulation of LC3B-I is the marker of autophagy inhibition. We feel confused to draw a conclusion.

How to explain these results? Can we do some more experiments to analyze autophagic flux?