How efficient can in vitro recombination in a gateway cloning system between the vector pDONR221 as the entry vector, with a promoter fragment subcloned of 5kb and the pBGWFS7 (12.4 kb) as the destination vector?
Yes PDONR221 can be used as entry vector entry gateway system
pDONR221 (12536-017): empty middle entry vector for generation of new middle clones.
empty 5' and 3' donor vectors for generation of new 5' and 3' entry clones. Note: the catalog number provided here is for the MultiSite Gateway Three-Fragment Vector Construction Kit; it includes pDONR221 as well as a destination vector (pDest R4-R3). This appears to be the only way to purchase the empty donor vectors at this point.
The Gateway cloning method, invented and commercialized by Invitrogen since the late 1990s, is the cloning method of the integration and excision recombination reactions that take place when bacteriophage lambda infects bacteria. This technology provides a fast and highly efficient way to transport DNA sequences into multi-vector systems for functional analysis and protein expression using Gateway at sites.
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