is excellent, working in our lab for more than 5 years, almost all days, without problems

Thanks for the advice. One other thing, do you know if you can run 10ul reactions in the 200ul tubes? The website says minimum of 20 but I wonder if less is still possible?

we never try less than 15ul

OK. but 15 ul is good in the 200ul tubes or are you using the 100ul tubes (these appear more expensive for some reason)?

we use 200 ul tubes or strips for 72 reactions

I am using two Qiagen Rotor Gene Q machines in my lab. One machine worked all fine, but the second machine had to be fixed many times. Otherwise, I am really happy with the results and technology, especially melt curves.