What type of regression should I be using?

I am planning to conduct a study with the following variables: A - independent variable B - intervening variable C - dependent variable D - moderating variable between B and C The framework...

22 October 2020 5,324 3 View

Statistical test with control and three treatment groups?

Hello everyone! I am working on some feedback, and someone suggested that I run a replicated regression on my data (semi-quantified western blots mostly) instead of an ANOVA with a Dunnett...

04 August 2020 1,052 3 View

How can I identify a fungal species from a sample not isolated from a petri plate?

We have a brown fluffy fungus growing on plant material samples and we are not sure what it is. It also does not grow on a petri dish either so we are not able to isolate it. Is there a way to...

04 June 2019 3,105 2 View

How to decide which package is best for my thesis "SPSS" or "SEM"?

I am doing my thesis with a total of 206 samples. I am a bit confused about what software should I use to analyse the data; either SPSS / SEM? And why should I use that? My lecturer suggests...

08 May 2019 1,840 4 View

Suggestions for a non-toxic UV reflecting white paint or alternative?

Hi all, I'm looking for a non-toxic UV reflecting white paint or alternative to paint a range of clay model morphs of moths that I will put in the field to record survival statistics. The models...

26 February 2019 8,902 1 View

How to analyze data with one IV and 3 ordinal DV's at pre, mid and post test?

Hi, I'm researching a treatment and using the CMAI short form as a measure of participant agitation. The CMAI short form breaks questions into 3 categories of agitation. I administered the CMAI at...

21 November 2017 5,284 3 View

Quick way of measuring mitochondrial dysfunction?

Our lab is looking to measure mitochondrial dysfunction in spinal cord tissue. We think that a drug is reducing mitochondrial damage normally caused by experimental autoimmune encephalomyelitis....

30 October 2017 8,977 3 View

How much of meat in major countries ie: USA, China, UK, Australia, Europe is from factory farms compared to naturally reared or free range farms.

Research on animal agriculture.

31 January 2017 8,490 2 View

Has anyone administered hormones to mice through the use of creams?

I am looking to administer estriol over a long period of time (more than a year) and am considering the use of topical creams. Has anyone used this method or know of a paper or lab that has used...

22 November 2016 9,070 3 View

Can anyone confirm if Litchi and Strychnos seeds are endospermic?

Both seeds are recalcitrant and have relatively small, thin cotyledons that do not appear swollen, so I assume that that the surrounding tissue is endosperm but cannot be certain.

26 October 2016 7,784 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Is my GFAP staining in right position?

I was differentiate hESC to astrocyte. In the second week of differentiation, I stained my cell with s100beta and GFAP. I expected that the staining site of GFAP would come from the cytoplasm,...

15 February 2021 8,982 1 View

How can I calculate volume fraction of MWCNT from a nano hybrid composite ?

I am adding 0.1wt percent MWCNT to glass fiber epoxy composite and want to determine the volume fraction of the MWCNT from this composite but I don't know how to calculate it. can anyone help me...

09 February 2021 8,883 3 View

Fractional derivative users, are you on the same page with me on the validity of the fractional order parameter, say alpha?

Taking a fractional ODE problem as an example. It has come to my attention that if the fractional order parameter is in the interval (0,1], many only presents results for alpha=0.9, 0.99. I saw...

08 February 2021 9,671 3 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Is there any protein soluble in oil?

Hello, I am looking if proteins or protein fractions soluble in oil exist? For food application Thank you for your help

01 February 2021 2,103 3 View

How best can one separate n-hexane-soluble fraction of plant extract that binds strongly to silica gel columns and what are the possible compounds?

I wanted to elute n-hexane-soluble fraction of a plant extract in a silica gel column using different proportions of dichloromethane/n-hexane. The bands separated visibly initially but moved only...

18 January 2021 9,270 3 View