Is prostatic acid phosphatase present in blood?

14 March 2019 1 6K Report

We know that prostate secretes various vitamins and proteins including prostatic acid phosphatase into seminal plasma.

But I was wondering if some of these ( particularly prostatic acid phosphatase) is secreted into/comes in contact with blood.

Wayne Michael Butler

For nearly 30 years, we have been measuring serum prostatic acid phosphatase as part of our clinical staging workup of all new prostate cancer patients. PAP has a very high negative predictive value for a nuclear medicine bone scan result, so we risk stratify patients to avoid bone scans is most patients. I've attached two of our papers on the usefulness of the test.

Badges
Science topic

More Satabdee Mohapatra's questions See All

I need MD simulation in 500 ns for some of my test compounds with PBSA, GBSA and PCA. If a researcher will help, we will provide authorship?

For my research, I need MD simulation of 500 ns with PCA. Please reply if you have facility. We will provide corresponding authorship. The article will be published in a high IF journal. My email-...

26 November 2023 6,926 0 View

How to increase the solubility of chlorophyll extracted from plants for bioimaging studies?

Please provide information related to any coating that can be done or any composites that can be synthesized.

09 October 2023 9,003 2 View

As we all know that Si 2p peak can be deconvoluted into two peaks such as Si 2p1/2 and Si 2p3/2. Which peak has higher binding energy and why ? ?

In the few literature, it was mentioned that the binding energy of Si 2p1/2 was higher than Si 2p3/2, while some authors made the just opposite statement about these peaks. I am so confused about...

22 June 2023 1,135 2 View

What is the JCPDS No and CCDC no for Ag-MOF-5?

Ag-MOF-5 synthesized from Terepthalic acid, Silver Nitrate and DMF. Need to know Chemical formula, Molecular weight and molecular structure of Ag-MOF-5. Thank you

21 May 2023 1,624 0 View

Can a composite show different dual phases (space group) in room temperature XRD and room temperature Neutron diffraction Reitveld refinement?

If possible kindly suggest some references.

05 February 2023 3,683 3 View

How to isolate fibroblast cells from hamster?

I isolated fibroblast cells from hamster by collagenase A digestion method. I used DMEM with extra L-glutamine as culture media. After 24hr there is no attachment of cells. The flask was coated...

28 November 2022 4,816 1 View

Which is the best technique- FACS or MACS for separation of dead cell from live cell population by using PI and SYTO9 fluorescence dye?

I want to separate dead cells from a bacterial population after treatment of any drug or antimicrobial agent. Which technique will be more efficient FACS or MACS if I use PI and SYTO9 dye ? Is...

15 August 2022 4,009 3 View

How to separate my metal microcage synthesised in ethylene glycol and water solvent ??

I tried to pellet it down at a maximum of 18K rpm, but it did not work. I heated the reaction mixture up to 200 C temp to evaporate the Ethylene Glycol(Including water) and to get the metal...

21 June 2022 6,740 0 View

Can anyone please help me with a protocol for in-vitro phosphorylation using CAMK2α ?

I want to phosphorylate my protein using CAMK2α. I ordered CAMK2α Kinase Enzyme System from promega...

06 June 2022 5,693 0 View

Is it possible to cryopreserve the Neonatal primary cardiomyocyte?

Neonatal cardiomyocyte is the sensitive primary cell line. Can a researcher cryopreserve the cell line for future experiment?

30 December 2021 4,160 3 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View