Does PVP (polyvinylpyrrolidone) percentage also play A vital role ex 7 % PVP or 10 % PVP or 12 % PVP?
More % of PVP more fragmentation in embryos or vice versa. Do you have any publication OR datasets to support.
Subhash C. Juneja ·:Why someone wants to add additional PVP and think about this. Need to add minimum possible to help handling (non-sticking to surfaces) (not to lose embryo). Anything in excess may cause at least deprivation of embryos for the access to good media rather than loaded PVP with media.
People were doing ICSI without any PVP earlier. Companies come up with product to make the things easy like 10% PVP not knowing what will be the effect on new born baby in long-term. I will try to avoid PVP or not more than 1% PVP so that oocyte and sperm does not stick to surfaces. Remember, with ICSI, along with sperm head, 10% PVP gets into egg.
ON the other hand, I doubt PVP does affect pH.
More experts in the field can put some input to your question,
Regards,
Subhash
Josep Santaló:
Sorry but I cannot agree: To my knowledge PVP does affect pH. Low molecular fragments of PVP that are mixed with appropriate size molecules (30-40 KD) tend to be acidic. That's the reason why PVP is dialized when preparing: to remove these fragments and stabilize pH.
Josep
Murid Javed:
In human ICSI, 7% PVP is commonly used. Earlier studies showed some toxic effect due to impurities. Medical grade PVP is considered safe now. For your information some of the older publication are given below. There is another physiological alternative to PVP as well.
Theriogenology. 2009 Sep 15;72(5):624-35. doi: 10.1016/j.theriogenology.2009.04.018. Epub 2009 Jul 14.
Effect of PVP on sperm capacitation status and embryonic development in cattle.
Kato Y1, Nagao Y.
Hum Reprod. 2003 Sep;18(9):1887-9.
An alternative to PVP for slowing sperm prior to ICSI.
Balaban B1, Lundin K, Morrell JM, Tjellström H, Urman B, Holmes PV.
Hum Reprod. 2002 Sep;17(9):2350-5.
Fertilization and embryo development in a mouse ICSI model using human and mouse sperm after immobilization in polyvinylpyrrolidone.
Mizuno K1, Hoshi K, Huang T.
Intracytoplasmic sperm injection with polyvinylpyrrolidone: a potential risk.
Jean M, Mirallié S, Boudineau M, Tatin C, Barrière P.
Fertil Steril. 2001 Aug;76(2):419-20. No abstract available.
Influence of polyvinylpyrrolidone on the outcome of intracytoplasmic sperm injection. Tsai MY, Huang FJ, Kung FT, Lin YC, Chang SY, Wu JF, Chang HW.
J Reprod Med. 2000 Feb;45(2):115-20.
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