Can anybody translate the reference section of D. Kovachev "Effect of PHA ...Paramecium...1980, journals.mu-varna 17 & where is ref 8?

I'm writing an article and need translation and reference number 8.

06 July 2018 2,318 6 View

Does anyone know of research where phytohemagglutinin was used on protozoa, especially protozoan parasites.?

My reason for asking this question has to do with the extreme difficulty that many humans have in resisting malaria. I suspect that part of the reason is that lymphocytes evolved from protozoa and...

10 November 2017 987 3 View

Loops on outside of pressure hose

07 August 2017 6,375 3 View

No title

03 March 2021 5,360 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

MHC Rabbit Genome Annotation? Domain annotation of each MHC Rabbit Gene?

I want to find a database(s) or a method where I can find and bulk download all the rabbit MHC genes and domain annotation. In humans using Ensembl, all MHC genes have names and domains but in...

28 January 2021 7,722 2 View

How do I avoid artifacts during paraformaldehyde fixation of mouse CD8+ T cells?

I work with mouse OT-I CD8+ T cells which have been activated in vitro with peptide-MHC and B7 proteins for 3 days. I've been trying to standardize protocols to fix these cells using...

28 January 2021 8,519 2 View

Does re-stimulation of T cells with PMA and Ionomycine before staining for intracellular cytokines for flow cytometry affect reliability of the assay?

I understand that to be able to measure intracellular cytokines there is a need to restimulate with PMA and Ionomycin but I don't understand how the results will be reliable after that. PMA and...

18 January 2021 7,240 7 View

Can someone help me identify the bands?

The samples are replicates (4) of total RNA from HEK-293 T cells. The ladder is a 1kBp ladder on a 1% native agarose gel buffered with 1x TAE. From my searches, there should be two sharp bands at...

15 January 2021 4,712 4 View

Does anyone have problems performing CRISPR KO in human primary T cells?

For the past few months, I have been troubleshooting a CRISPR KO of human CD3e in human primary T cells. I got the KO to work one time using 1250 ng TrueCut Cas9 (Thermo) + 250 ng (or 7.5 pmol)...

11 January 2021 6,016 3 View

Do murine T-cells get transient activation when they attach to a tissue culture treated plate?

I have been trying to activate CD19 CAR-T cells using plate-bound anti-CAR Abs. I am getting activation both in cell lines and primary CD8 T-cells. I am using pErk as a readout for activation. I...

30 December 2020 1,655 2 View