Is perforin 2 present in NK & CTL vertebrate lymphocytes?

Alan Coulson @Alan_Coulson

01 January 2019 0 1K Report

Every reference to perforin in lymphocytes talks about perforin 1.

Has any one demonstrated the continued presence of perforin 2,

the original perforin, in vertebrate lymphocytes?

Badges
Science topic

More Alan Coulson's questions See All

Can anybody translate the reference section of D. Kovachev "Effect of PHA ...Paramecium...1980, journals.mu-varna 17 & where is ref 8?

I'm writing an article and need translation and reference number 8.

06 July 2018 2,508 6 View

Does anyone know of research where phytohemagglutinin was used on protozoa, especially protozoan parasites.?

My reason for asking this question has to do with the extreme difficulty that many humans have in resisting malaria. I suspect that part of the reason is that lymphocytes evolved from protozoa and...

10 November 2017 1,133 3 View

Loops on outside of pressure hose

Why don't they incorporate loops on outside of pressure hose to facilitate putting them on and removing them?

07 August 2017 6,539 3 View

An animal in not a rock (e.g., Koch 2013), it has an internal constitution that can be modified?

Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...

07 August 2024 648 0 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to transfect circular mRNA to T cells?

Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...

21 July 2024 4,322 1 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

How to culture and stimulating hamster splenocytes for T-cell analysis and ELISPOT?

Hi, Does anyone have experience and success of isolating, culturing and stimulating hamster splenocytes for T-cell analysis (FACS and ELISPOT)? I am currently using a protocol that we routinely...

09 July 2024 6,336 1 View

How to maintain intestinal tissue resident cells (TRM) in culture?

I am attempting to culture tissue resident T cells (TRM) derived from fresh intestinal tissue for up to six days. I am finding that the viability of the T cells is low at D0, and a very small...

08 July 2024 4,887 2 View

Suggestions for storage of CD4+ naive splenocytes for future use?

Hi! I am working on a biophotonics project in which I need isolated but inactive CD4+ splenocytes. Every protocol and piece of literature I have found immediately activates and cultures...

02 July 2024 4,887 0 View

Interpretation of bulk RNAseq and cell culture data?

Hi, I'm analyzing some bulk RNAseq data from T cells under different conditions, and based on the RNAseq data, condition A shows higher expression of genes associated with effector functions...

01 July 2024 741 2 View

How to set up Mouse neutrophil - T cell coculture in vitro?

Hi, scholars. I am planning to conduct mouse neutrophil and T cell co-culture in Vitro and observe the effect of neutrophils on T cells. I don't have any protocol. Could anyone provide me...

19 June 2024 3,271 2 View