Can agrobacterium tum. GV3101 equally grow with both peptides? What is the difference between them?
I want to know more about Uranium ore deposits in world.
11 August 2024 6,720 0 View
I want to know more about diamond ore deposits in world.
11 August 2024 2,167 1 View
We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
10 August 2024 6,678 14 View
If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
10 August 2024 8,198 5 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,229 0 View
What are a “Farmers Producer Organization” (FPO) and its essential features?
10 August 2024 477 5 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...
08 August 2024 4,831 4 View
How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
08 August 2024 8,162 0 View
Hi all, I have been facing problems with colony pcr for agrobacterium colonies. I did site directed mutagenesis, cloned to pcambia, sequenced and confirmed and then transformed to Agrobacterium....
02 June 2024 6,517 5 View
Hello all. I transformed some Agrobacterium competent cells by electroporation and performed colony PCR to see the positive transformants. However, I performed the glycerol stock after 10 days of...
26 May 2024 9,304 3 View
I am confused regarding the desired edit efficiency of PEP2 for transversion on rice plants using Agrobacterium.
21 May 2024 4,332 0 View
Strain: LBA4404 Growing media: YEB (pH 7) Temperature: 28°C, 200 rpm (overnight culture) Antibiotic use: Kanamycin and Streptomycin
13 May 2024 7,726 2 View
I could've sworn that I read a protocol where strained plant "juices" were added to agrobacterium cultures in lieu of pure acetosyringone. If you have heard of anything like this, please let me...
06 May 2024 9,001 0 View
Hello all. I purified some plasmids after doing precultures of strains from a 2-month old plate in -4 degrees. The PCR results were fine and I used theses plasmids to transform Agrobacterium...
26 April 2024 8,440 3 View
In case of Thraustochytrids, I got more than 6 isolates by agar plating. However except Aurantiochytrium spp. all others grow very slowly. What could be the reason behind? I am using GYP broth...
05 April 2024 7,486 2 View
Hello. I am trying to culture Agrobacterium GV 3103 and EHA105 in 15 mL LB media with the antibiotics kanamycin and rifampicin (100 ug/mL & 20 ug/mL)(added 200 uL of previously normally...
25 January 2024 812 5 View
We tried to do activation-tagging in Arabidopsis using the plasmid pSKi015. Nevertheless, we failed to obtain positive clones in GV3101? The recommended Agrobacterium strain for pSKI015 is GV3101...
21 January 2024 1,246 2 View
I need some strains of Agrobaterium rhizogenes
02 December 2023 1,549 0 View