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I wish to find whether someone is having a positive history of using Pen/Strep (at 1%) for in vitro culture of Plasmodium. we have been using Gentamycine, which looks safe for the parasites but...
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We are planning to start in vitro work with Trypanosoma brucei. But we have been facing some challenges in culturing from cryopreserved vials, using Iscove’s Modified Dulbecco’s Medium (25 mM...
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Protein supplements (Human serum & Lipid-rich albumin) are all limited by their cost. An ideal solution could be to have a cheaper supplement like BSA capable of sustaining the parasite continuous...
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Bacterial and fungal contamination is is a major challenge in Parasite culture in general and Plasmodium in particular. Several antibiotics are efficiently used to prevent such contamination....
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I have come through a couple of publications claiming that simple MTT could replace the Lactate Dehydrogenase Assay in malaria drug susceptibility testing. MTT is quite cheap compared to LDH kit,...
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