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Questions related from Denis Zofou
I wish to find whether someone is having a positive history of using Pen/Strep (at 1%) for in vitro culture of Plasmodium. we have been using Gentamycine, which looks safe for the parasites but...
06 June 2017 2,872 3 View
What are the cell lines which can be used in for anti-adhesive activity in salmonella drug discovery? Can LLC-MK2 also be used?
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Could some one advise me on a simplest and cheapest formulation for topical gels?
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We are planning to start in vitro work with Trypanosoma brucei. But we have been facing some challenges in culturing from cryopreserved vials, using Iscove’s Modified Dulbecco’s Medium (25 mM...
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Protein supplements (Human serum & Lipid-rich albumin) are all limited by their cost. An ideal solution could be to have a cheaper supplement like BSA capable of sustaining the parasite continuous...
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Bacterial and fungal contamination is is a major challenge in Parasite culture in general and Plasmodium in particular. Several antibiotics are efficiently used to prevent such contamination....
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I have come through a couple of publications claiming that simple MTT could replace the Lactate Dehydrogenase Assay in malaria drug susceptibility testing. MTT is quite cheap compared to LDH kit,...
15 December 2012 6,110 5 View
After screening of potential drugs in vitro, what is best way of determining the concentrations to use in animal model, like mouse model for example, using IC50 values?
17 August 2012 4,807 7 View