Is PBS a suitable vehicle for testing the hemolytic activity of drugs in Ex vivo red blood cell hemolysis assay?

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How can I write Peng Robinson equation with Volume explicit and Preasure constant?

I need to use Peng Robinson with Volume explicit to do the parcial derivative dv/dT with P constant of this equation.

17 April 2024 296 4 View

Electroporation parameters for jurkat cells using neon system?

Hello! I have tried to electroporate the Jurkat cell line with the Neon System using the parameters provided by the supplier, but almost all the cells die after the procedure. I have also tried...

10 April 2024 1,382 0 View

¿Cómo puedo informar correctamente aceerca de un posible caso de trata?

Todo el mundo alega que si ocurriese una injusticia lo correcto sería contarlo pero, cómo se debería dar voz de forma adecuada en este caso, sin poner en riesgo a las víctimas?

10 March 2024 5,202 1 View

¿Es lo mismo trata de personas que tráfico de personas?

Normalmente se suelen utilizar esos dos términos coloquialmente a la hora de hablar sobre el tema, y me gustaría saber las diferencias para llevar el tema con propiedad y que más personas sean...

10 March 2024 2,567 1 View

Is it possible to use the Emotiv Flex (either the Saline or Gel version) for Neurofeedback sessions?

Additionally, what software can be used for training that is compatible and easy to set up with this device? Thanks

16 January 2024 3,464 0 View

Why doesn't fsolve in Maple return all complex roots of a polynomial?

Why does fsolve(x - (-8.0)^(1/3), x) returns only the first root (1.000000000 + 1.732050807*I) of the polynomial (which clearly has 3 roots)?

28 December 2023 8,244 0 View

How can I explain a strange Kinetics behavier for oxidation using H2O2?

I'm doing some kinetics studies, and my reaction always shows a strange behavior, it increases over time the yield of products, reduces and than increases again, like a wave. I'm currently using...

05 September 2023 586 2 View

Does anyone, by any chance, have the datasheet from the HPLC column Pharmacia Sephasil Peptide C18 5u ST 4.6x250 mm?

Hello everyone! I recently decided to run some tests on an old HPLC C18 column but I'm unable to find its datasheet. Even though I know wich buffers to use, I really would appreciate working...

30 August 2023 8,368 2 View

Can anyone explain the morphology found in astrocyte labeling with GFAP?

Using the same antibody to mark astrocytes, we found markings with commonly known shapes, but we also found markings similar to the image below, both in the same animal and in other animals of...

17 August 2023 4,091 2 View

How to develop the Mander curve of a concrete column?

I need to perform the manual calculation but I am confused about the formulas and steps to follow for the elaboration of the Mander Model in a concrete column

18 June 2023 5,340 1 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View