Is PBS a suitable vehicle for testing the hemolytic activity of drugs in Ex vivo red blood cell hemolysis assay?

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Is there any blood pressure device/module/kit for IOT (digital sphygmomanometer)?

Hi RG, I'm looking for a blood pressure device/module/kit which outputs raw data (serial or wireless, digital or analog). Usually, the information from these devices is on-screen only or, if...

21 October 2020 9,576 5 View

Why doesn't my dbRDA show all the environmental variables considered?

I am performing a db-RDA in R using the package Vegan and the function capscale. I have taken into account 24 environmental factors but the program only considerate 7 of this factors. I have 54...

26 August 2020 919 6 View

What features should I extract from EEG signals?

Hello RG, I have been working with EMG signals and a common approach to reduce the dimensionality (in order to classify events for example) is to extract a set of features (in time and frequency...

25 May 2020 6,938 4 View

How to make the student participate in the virtual classroom during the COVID-19 pandemic?

We are facing a difficult problem during the pandemic: the number of students attending virtual classes has decreased significantly at the undergraduate level.

20 May 2020 5,122 27 View

Any suggestions on how to rinse out Na in my ICP MS instrument?

The Tune check report shows a large Na peak next to the Mg (mass 24) peak in the Resolution/Axis portion of the report. I have cleaned the nebulizer, spray chamber, connector tube and interface...

04 May 2020 7,776 1 View

How to identify data outlier in triplicates without using the methods of interquartile range and Z-Score? Can I do it for ANOVA?

30 April 2020 2,107 3 View

COVID-19 database?

I'm looking for a COVID-19 updated database containing patient information (age, sex, date disease was diagnosed, date of death/recover, etc). Is there any?

10 March 2020 5,565 14 View

Why do dispersed nanotubes have more intense peaks in the absorbance spectrum at (approximately) 260 nm?

Is there any physical or quantum explanation for this?

11 December 2019 8,317 2 View

How to analyze fcs flow cytometry files with multiple samples?

I am trying to analyze a single fcs file containing data from several samples but every time I open it in FlowJo X, Flowing Software or FCSalyzer I see all the acquired events instead of separate...

25 November 2019 5,963 3 View

How to retrieve a 3D model from a set of parameters using supervised learning?

I have a data base of 3D models along with a set of shape measures (length, diameter, etc). Is there some supervised learning method (metric learning, deep learning, dictionary learning) which is...

22 August 2019 3,223 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View