Is not cfDNA extracted using carrier RNA suitable for NGS experiments?

Dasom Kim @Dasom_Kim18

27 March 2019 1 4K Report

I heard that extracted cfDNA using carrier RNA is not suitable for NGS libaray prep.

is that right?

I want to extracted high concentration cfDNA using carrier RNA but also worried about failed NGS library..

Ben Abraham

Hey Dasom,

This is probably a little too late but there are cfDNA isolation kits that'll work without any carrier RNA. I've included a link below if you haven't found one yet (I hope you have).

Plasma/Serum cfDNA Advanced Purification Kit

https://norgenbiotek.com/product/plasmaserum-cfc-dna-advanced-purification-kit

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