Dear proteomic experts,

I just recently noticed on Metascape ''Note: gene lists containing more than 3000 genes are not analyzed. Please use a more stringent hit calling criterion, as you most likely have retained too many genes.''

Well, my gene list consisted of a tiny bit more than 3k proteins (roughly 3076 +/-). I still got enrichment analysis charts and everything.

Would you say, that the analysis went through by a hair and I can trust the graphs? Or were some of my genes omitted from the list, eg. the last ones that exceeded 3k? If so, the outcome would lack some genes...

Thank you all!!