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Questions related from Anja Krivograd
Looking for somebody to share experience on using ChatGPT 4 as an assisting tool (in combination with either Excel or Perseus MaxQuant) for analyzing data such as OMICs. Concretely I'm dealing...
30 November 2023 3,951 2 View
I suspect the protein I'm trying to detect is sensitive to SDS in my lysis buffer. Of course, I could shorten the lysis duration but it's not a good option for several reasons. Does anyone...
27 January 2023 4,040 2 View
Dear proteomic experts, I just recently noticed on Metascape ''Note: gene lists containing more than 3000 genes are not analyzed. Please use a more stringent hit calling criterion, as you most...
14 August 2022 9,895 0 View
I submit my accession codes for proteins as usual but this issue pops up: '' Due to database update, bug fix, and continuous features improvement, Metascape does not provide a mechanism to...
15 January 2022 1,839 3 View
I've been trying for months to detect my protein in Western Blot. Firstly there was an antibody problem, now I should have a perfectly good antibody which I want to test, but the protocol doesn't...
13 September 2021 5,830 6 View
For my 15kDa protein, what is better the 4-12% Mini Protean TGX gel from Biorad or 4-12% NuPage precast Bis-Tris gel? Is there a significant difference between the two? I'm doing the WB, could the...
29 June 2021 1,774 1 View
Hello there, I'm a bit rusty in statistics and would appreciate quick kick-start advice. I have my Mass spec data for over 3k proteins and 4 conditions in which they appear or don't appear. What...
28 May 2021 8,076 6 View