RNA species both eukaryotic and prokaryotic are generally considered fragile. I am wondering whether they could be stronger and resistant to denaturants like heat.
Hi, for a long period of heat exposure mRNA should be degraded but for some manipulations heat treatment at 65-70 deg for 15-20 min is ok.
Wilber, it very depends on what temperature ranges the mRNA is subjected to, indeed higher temperates and longer exposure times can speed up the degradation rate of your samples. It is quite difficult to specify which temperature ranges, but as hinted by Yordan, temperatures above 70 degs C For time frames longer than 20 mins can result in some degradation.
Thank Yordan and Jimmy for the thoughtful answers. I think you might have touched on the answer but in question I am referring to mRNA within the cell. In other words whether heating up bacterial cells to temperatures above 80oC would destroy the intracellular mRNA?
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