Dear scientists,
It is clear that madcam-1 is expressed in human colon and small intenstine endothelian cells?
Does anyone have reliable info regarding madcam-1 expression on human esophageal and stomach endothelian cells?
thank you
I am interested does anyone tried to measure tree rings from sub-fossil oak cross-sections using an image analysis technique? When working with tree-ring measuring table, I usually do the...
27 March 2023 4,264 2 View
Dear Collegues working in the field of bone marrow transplantation, Could anyone with experience suggest protocol of conditioning regimen for multiple sclerosis and systemic scleroderma before...
22 June 2016 1,064 3 View
I would like to know your suggestions regarding human bone marrow aspiration for mesenchymal stromal cell expansion. What should the ACDA:MSC ratio be in a syringe (to avoid clotting in syringe).
24 October 2013 209 1 View
I am looking forward to checking for molecules (CD) expressed on activated human BM-MSC after IFN gamma +/- TNF alfa stimulation. CD73, CD90, CD105 plus which other molecule/-es? CD274? CD54?...
23 September 2013 740 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View