14 Questions 4 Answers 0 Followers
Questions related from Saesha Verma
I have a consortium of agar-degrading microorganisms on the f/2 agar plate, and they have somewhat liquidated the agar. Now I want to know how to collect the bacterial culture from the plate and...
08 January 2025 909 1 View
I am working with phototrophic biofilms and would like to cryopreserve them for future experiments. My primary goal is to maintain the viability and structural integrity of the biofilm, as well as...
08 January 2025 9,936 0 View
I want to do microbiome analysis using R, for which I have three folders that contain ASV data, metadata i.e. sample data, and taxonomy data for 56 sample studies. I want to read the data in those...
20 May 2024 5,141 2 View
I have 10 pre-processed studies for which I have prepared ASV tables, Taxa tables, Metadata, and phylogenetic trees. Now I want to merge these studies and create and single or merged phyloseq...
18 April 2024 7,221 1 View
@SRR8393705.1 1...
12 April 2024 6,074 0 View
The issue is related to illumina clip command. Error: Exception in thread "main" java.lang.NumberFormatException: For input string: "/Users/Saesha...
09 March 2024 5,103 2 View
When I write this command to generate the ASV table write.csv(seqtab31.nochim, file = "ASV_table31.csv", row.names = FALSE) The file is not able to open "The text file contains more data that...
25 October 2023 5,630 0 View
SRR no. input filtered denoisedF denoisedR merged Nonchim % drop SRR15372855 210711 186522 175583 176162 116563 36509 83% SRR15372856 209697 183184 173837 174328 127588 38205 82% SRR15372857...
22 October 2023 9,109 3 View
I'm encountering an issue while trying to read a file named 'Meta_31.txt' in R. Despite confirming the file's existence, verifying the file path, and trying various methods (e.g., read.table,...
04 October 2023 6,293 1 View
I have to pre-process around 10,000 fastqQ files containing 16S rRNA sequences. According to the multiQC reports generated before pre-processing the sequences have good quality reads with Q score...
18 August 2023 2,630 3 View
Error message- “An error occurred during processing. A report was generated into the file '/home/poorna/ncbi_error_report.txt'. If the problem persists, you may consider sending the file to...
07 August 2023 1,730 1 View
Every time on reopening the terminal in the Linux system, it states that the fastq-dump command is not found. Then I repeat the entire downloading process again and again.
07 August 2023 3,278 3 View
I have downloaded fastq files but when I open it black screen appears. Is there any specific software or command for viewing the fastq files? I think it is opening in the text editor in Linux system.
25 July 2023 10,078 2 View
I have many SRA accession numbers and I need to download the fastq files. I am using fastq-dump command to download the fastq files in the Linux system but it works for a single accession number....
21 July 2023 287 3 View
