Is Lipofectamine 2000 available to transfection antibody to HeLA cell? or just RNA and plamid DNA only?
Hello Adrian,
DNA and RNA are negatively charged, as is the plasma membrane. Due to this there is electrostatic repulsion of nucleic acids, which reduces transfection. Lipofectamine is cationic and helps to overcome the electrostatic repulsion, thus increasing transfection effeciency. The major barrier to penetration of antibodies is their size. It is not likely that lipofectamine will be of much use in this instance. However, for lipofectamine to be of use the protein would have to be negatively charged to allow complexing with the lipofectamine. You may want to refer to a recent report in Nature Biotechnology (Nature Biotechnology 33, 73–80 (2015) doi:10.1038/nbt.3081).
Hope this is helpful
Thanks for your help, Mr. Yazad.
should we use antibody delivery-in to transfection antibody to HeLa cell? or do you have any other suggestion?
What is your application? Are you using the antibody for labeling in fixed cells or do you want to deliver it to living cells?
Hello Adrian,
I Agree with Mr Yazad, the electrostatic repulsions and the Ab size are making things difficult for antibody delivery. However OZ biosciences launched recently Ab-deliverIN for functional antibody delivery into living cells. Few publication have already been made with it, and they are offering free samples.
I hope this will help,
Best,
Romuald
http://www.ncbi.nlm.nih.gov/pubmed/24848507
http://www.ncbi.nlm.nih.gov/pubmed/25662860
Hello Adrian,
You may want to try Chariot protein delivery reagent. We have used it with satisfactory results.
https://www.activemotif.com/catalog/37/chariot-protein-delivery-reagent
- Badges
- Science method