Or do I need to perform several cross between CMS and restorer lines with different genotypes?
It s extremely difficult to map restoration as you have to do test crosses so handling more than one cross would require a lot of resources. Unfortunately for peer reviewed publication you may need replication. Personally I would try in one cross and if I found a linked marker would test ii in a subsequent cross or crosses.
Hi Anik,
I would not stick to a single cross bit would definitely investigate several combinations with at least different restorer lines if available. You will reinforce the consistency of you data in addition and as underlined by the answer above if you need to publish.
Good luck,
Pierre
Hello Pierre and Witcombe,
The problem is I have to complete my research in 3 years and I also need to consider the budget for phenotyping and genotyping. I am thinking about crossing one CMS line of wheat to two different restorer lines with more than 90% restoration ability. Then I will have two F1 population and I will generate like 300 F2 population as my mapping population. Then I will do genotyping. After tha,t I will have phenotypic and marker data for QTL study. So, how valid and efficient will this procedure be?
Hi Anik,
I understand your concern, budget and time have to be considered of course! Two F2 populations sound fine to me and definitely more robust that a single one. The procedure seems also fine. Too bad that you don't time enough to generate RILs or DHs as mapping populations if there were possible to get in your species. Is that wheat you are dealing with as you are from Hohenheim :-)? Best regards and good luck!
Pierre
Agree. If its for a PhD you have to do a minimum of two otherwise the external examiner will be asking why you did not replicate. My answer was from a resource efficiency viewpoint not from doing a PhD. Ignore my remark about test crosses as the way you propose to do this avoids the need - I assume you will look for fertility restoration in the F2 plants. Good luck.
Hello Witcombe,
I am looking for a QTL that restore the fertility in the F1 as I look forward to establish a stable hybrid production system.
Hello Pierre,
Yes it is wheat.
hello Pierre,
What would happen if cross a CMS line to a restorer in two different environment, 1) field condition 2) green house? I will get 2 F2 population then. Will it be a good idea?
Hello Pierre,
If I use two environment to generate 4 F2 population from two crosses, then do I need to genotype every F2 population or I can use them for comparison of phenotypic data?
Please tell me.
Two environment is different therefore phenotype would also differ. If it is quantitative traits I will say not possible. If it is major gene least influenced by the environment you can do.
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