Anik Dutta

15 Questions 18 Answers 0 Followers

Questions related from Anik Dutta

Tools for SNP imputation?

Hello, I am working with polyploid plant species (tetraploid, hexaploid, octaploid). I have genotype by sequencing (GBS) SNP data from different sequencing platforms like Affymetrix and SeqSNP....

07 July 2023 5,366 0 View

Why there is only few GWAS done on plant pathogen virulence or aggressiveness?

As you know GWAS have become very popular for finding causal variants using natural populations, then why there is a huge discrepancy between the number of GWAS done on hosts (wheat, maize, rice...

01 November 2018 3,377 1 View

Which software to use for trimming sanger sequenced data, making contigs and mapping to reference gene?

Hi, I have an amplicon of 1.6kb and I have sequenced it using forward and reverse primer. Now, I have to trim both the sequences and assemble those into one contiguous sequence. My goal is to...

04 September 2018 3,102 6 View

How to keep track of latest publications in specific research areas?

Hi, I would like to know how can I set up alerts for latest publication specific to my research field? My research interests are in wheat genomics, GWAS, wheat genetics, Zymoseptoria tritici,...

05 July 2018 1,783 3 View

How to anchor wheat 15K SNP array to the reference genome to obtain marker physical position and chromosome number?

Hi I have my snp dataset from 15K wheat SNP array. Now I want to anchor those markers to the latest wheat reference genome to obtain marker position in bp and chromosome number. Can someone tell...

27 April 2018 2,937 2 View

Which experimental design is suitable for conducting GWAS?

Hello, I have a large accession number (n=200). I will screen them for blast disease resistance and then conduct the GWAS. I will conduct the experiment in greenhouse where I have limited space....

23 May 2017 5,897 8 View

How to obtain uniform fungal spore spraying on plant leaves?

I want to test 96 genotypes planted on a single tray (96 small pockets, 3 seedlings per pocket) for blast disease resistance. I will spray spore suspension on 2 weeks old seedlings. I want to...

15 May 2017 5,931 13 View

Does it make sense to get heritability of zero?

Hello, I am working with maize drought tolerance. I am analyzing data under drought stress and normal condition. While doing data analyses I have found that traits like Anthesis silking interval,...

12 April 2016 9,448 10 View

What kind of problems may arise if I do not transform my data?

I have the data of maize drought trials. There are some traits for which data were collected based on scores. For example, 1=severer rolling, 5=no rolling. So, do I need to transform these kind of...

23 March 2016 4,684 2 View

A very basic question about molecular marker: how to develop a marker for a trait?

Suppose, I want to find a marker for drought tolerance in maize which nobody has identified yet. So, how can I develop that marker so that in future it can be used by others to screen populations...

17 January 2016 4,772 10 View

Is it wise and scientific to perform only one cross between a CMS line and a restorer line to find the genes restoring fertiliy in the F1?

Or do I need to perform several cross between CMS and restorer lines with different genotypes?

30 November 2015 6,175 9 View

Can anyone tell me the procedure of single marker analysis with DArt markers to find QTL?

I want to find QTL for fertility restoration. So, I want to do a classical QTL study using DArT technology and I want to integrate the result of single marker analysis with that. Please help me...

29 November 2015 4,275 5 View

Hi, can someone tell me how to characterize cytoplasmic male sterility in a crop?

In case of finding new restorer loci, is it necessary to develop new CMS line? Or one can use existing CMS line and cross with different inbred lines to establish a CMS system?

22 November 2015 9,341 1 View

Can someone give me any paper of CMS sources of wheat and how to identify new restorer loci for future breeding purposes?

I need to know how can I identify new restorer loci for hybrid breeding and which cytoplasm provides male sterility in wheat.

01 November 2015 7,013 6 View

How can I compute haplotype number and haplotype diversity from some given sequences in R with the package-"pegas"?

Suppose i have the following sequences and I want to calculate haplotype number and diversity using R-pegas. What code should I use? Sample 1 GATCGAACAG Sample 2 GAACGAACAT Sample 3 TATCGAACAG...

15 May 2015 7,079 2 View