Is it safe to transduce with lentivirus if they have already been transduced with sendai virus?

16 September 2013 1 2K Report

We would like to transduce some cells with lentivirus. But these cells have been transfected with Sendai virus. Is this feasible / recommended? What are the major points we should consider before proceeding with this?

Christian Praetorius

The major point to consider is the safety. Is it possible, by any chance, that you produce virus particles in these cells, that are infectious?

If not, is it possible, that the two virii interfere with each other?

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