We would like to transduce some cells with lentivirus. But these cells have been transfected with Sendai virus. Is this feasible / recommended? What are the major points we should consider before proceeding with this?
The major point to consider is the safety. Is it possible, by any chance, that you produce virus particles in these cells, that are infectious?
If not, is it possible, that the two virii interfere with each other?
I am trying to analyze lipids in plants; specifically pollen by using Sulfo-Phospho-Vanillin (SPV) Method! Can I prepare a standard curve for this estimation using soybean, sunflower, or olive...
01 July 2024 3,267 1 View
1. I was shocked to discover yesterday that my research citations on Google Scholar had dropped to 2383 from 2419 a week ago, and they are now up to 2391. 2. My citations three months ago were...
28 June 2024 2,846 2 View
Hello everyone, I am currently conducting research on the Oxygen Evolution Reaction (OER) mechanisms in perovskite materials and am particularly interested in studies that utilize Density...
03 June 2024 6,295 0 View
I wanted to express protein in mammalian cells for purification I need 6XHis tag, however, plasmid synthesis company have vector with Myc and his tag. So do I need both or only his tag.
12 May 2024 941 4 View
I needed a detailed methodology for SEM-EDS analysis for Leaf and pollen!
05 May 2024 4,892 5 View
Hi all, I'm looking to mass mark Solenopsis xyloni ants during an experiment. For this experiment, I'm allowing the whole colony to feed on three feeders, and I want to mass mark all the ants...
13 April 2024 9,988 2 View
Hey, I am doing an research on how parenting styles create negative attachment styles in young adults. I can't seem to understand what's the criteria for assessing and how to use it. It would be...
02 April 2024 7,017 2 View
Please share the details of the bonitur scale, its significance, and its relevance in current times.
01 April 2024 2,523 0 View
Hey all! I am looking for help to increase the citation count for my memristor test research. Memristor test is a relatively new area with limited commercial availability for these nanoscale...
27 March 2024 2,373 2 View
Has anyone run slide in Visium of tissue sections (Heart, kidney, brain) with CMC than OCT for spatial transcriptomics ???
14 March 2024 243 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View