What are the different way to give psychological (mental) stress in human population studies ?

01 February 2015 1,586 4 View

How to measure total body reaction time in human ?

I wan to measure total body reaction time in human

01 February 2015 394 0 View

Is there any possibility to differ auditory and visual reaction in different geographical region of same country ?

31 December 2014 7,464 3 View

Is it necessary to do GC-MS study for using crude extract to treat animals?

09 October 2014 2,834 8 View

What is the importance of ( df and F ) in one way ANNOVA analysis?

09 October 2014 2,971 4 View

What is the best method to isolate specific compound from plant extract?

09 October 2014 3,927 6 View

Can any one help me get a protocol for giemsa staining for bone marrow of wistar albino rats?

I want to do giemsa staining for bone marrow cells.

09 October 2014 8,393 5 View

Can anyone help me to get a protocol for cresyl fast violet staining for neuronal tissue?

I want to do cresyl fast violet staining for brain of wistar albino rats.

09 October 2014 550 1 View

What is the criteria for fixed dosing in vertebrates for any plant extract studies?

I want to know what the criteria should be to monitor the fixed dose for plant extracts on vertebrates (fishes).

08 September 2014 9,849 1 View

Can I use a zebra fish model to find out the brain antioxidant activity of aqueous extract of some fruit seed?

08 September 2014 8,225 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View