Is it possible to use qPCR to measure the effect of antimicrobial agents? Is it better then plate count.

More Karthikeyan Ramalingam's questions See All

Who the best teacher in your life and why?

In your school, or college, or university, or research institutions: who is best teacher in your life and why?

09 October 2013 8,179 19 View

Can someone give me their thoughts regarding minimum inhibitory concentration (MIC)?

For the new antimicrobial development… As per standard methods, what is the inoculum size one needs to use, in terms of optical density or CFU? What percentage of inoculum does one need to use...

05 June 2013 8,554 3 View

Antimicrobial agent’s assays?

Do you know any new assay for antimicrobial agent’s development? These are all in-vitro assays I am doing for antimicrobial agent’s development? 1. MIC, MBC, Disc diffusion, Well...

10 November 2012 1,703 1 View

Which one has more bacterial numbers planktonic or biofilm?

I am proceeding to do qPCR to know bacterial copy numbers in planktonic and biofilm (72 hours old biofilm with frequent media change) sample. Which one show more copies of DNA planktonic or biofilm?

09 October 2012 8,466 10 View

Does anyone have suggestions regarding the preparation of antibody-tagged nanoparticles for antimicrobial therapy?

I am working on antimicrobial activity of nanoparticles, for targetted antimicrobial therapy I am looking some help on antibody tagged nanoparticle preparation.

09 October 2012 1,984 8 View

I have chapter ready, Possible to add?

Dear Prof. I have chapter ready, Possible to add? Tentative title: Bioremediation- A logical approach for the efficient management of textile dye effluents. thanks for help..

01 January 1970 1,146 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why does the MFDFA algorithm need to calculate the profile of the time series?

As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....

05 August 2024 9,366 2 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

Is there any machine to do real time pcr?

I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering

05 August 2024 1,660 0 View

Juan Bueno

Dear Karthikeyan, is possible, there are currently Automated Quantitative Polymerase Chain Reaction as Xpert MTB/Rif. But the only problem is that the efflux activity contributes in some case with the resistance without need of mutations in any of the genes associated in the antibacterial target, for that reason in these methodologies clinical isolates can be classified as susceptible but are phenotypically resistant

http://jac.oxfordjournals.org/content/54/2/538.full.pdf

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0027689

http://jac.oxfordjournals.org/content/56/6/1000.full.pdf+html

http://www.ncbi.nlm.nih.gov/pubmed/21871582

Karthikeyan Ramalingam

thanks Bueno for the help

Pradyot Prakash

Dr Bueno indicated the role of efflux pump in phenotypically resistant MTB isolates. This is the reason why XpertMTB/ Rif ( qPCR) is being used only in those patients under National TB program (RNTCP) in India, who are clinically and microbiologically (smear positive) non responsive to first line of TB treatment.

I did qPCR measurement control and treatment both groups showed same CT value and may it need more serial dilution of DNA sample (my final DNA only 50ng/ul, I diluted 100 fold). I feel plate counts give better results then the qPCR, because qPCR more expensive, more work and more inaccuracy for antimicrobial agents testing. anybody has any thought on this... pls let me know...