Please be more specific;what nanoparticles you are exactly working on? The question of antibody tagging strategies would be based on that..

silver and gold nano particles for ESKEPE pathogens

Use thiol based capping ligands with carboxy/amine functional group;couple with antibody using carbodiimide chemistry

Alternatively you can synthesise the nanoparticles via standard reduction(borohydride for silver and citrate for gold) and add the antibody solution for direct capping by the antibody Cys residues.

Thanks, you have any paper for this, also I am going to buy the nanoparticles from outside

Dear Chowdhury,

Antibody tagging possible for all the nanoparticles (Fe, Cu, Sn, Zn, Ag and Au) and also I am looking to use metal nanoparticles not chemical derived one.

thanks

Karthi

What type of bacteria you intend to use, and what type of antibodies?

ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species)