Who the best teacher in your life and why?

In your school, or college, or university, or research institutions: who is best teacher in your life and why?

09 October 2013 8,112 19 View

Can someone give me their thoughts regarding minimum inhibitory concentration (MIC)?

05 June 2013 8,509 3 View

Which one has more bacterial numbers planktonic or biofilm?

I am proceeding to do qPCR to know bacterial copy numbers in planktonic and biofilm (72 hours old biofilm with frequent media change) sample. Which one show more copies of DNA planktonic or biofilm?

09 October 2012 8,415 10 View

Does anyone have suggestions regarding the preparation of antibody-tagged nanoparticles for antimicrobial therapy?

I am working on antimicrobial activity of nanoparticles, for targetted antimicrobial therapy I am looking some help on antibody tagged nanoparticle preparation.

09 October 2012 1,947 8 View

Is it possible to use qPCR to measure the effect of antimicrobial agents? Is it better then plate count.

If yes, could anyone please explain what are the factors I need to consider? Apart from control and treatment, what do I need to do for the calculation or final results?

09 October 2012 159 4 View

I have chapter ready, Possible to add?

Dear Prof. I have chapter ready, Possible to add? Tentative title: Bioremediation- A logical approach for the efficient management of textile dye effluents. thanks for help..

01 January 1970 1,106 1 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View