Is it possible to use of S1 nuclease instead of P1 for genome methylation analysis (lc-ESI-Ms/Ms based)?

Andrés Hernández-Pridybailo @Andres_Hernandez-Pridybailo

12 December 2016 1 8K Report

Hello,

I would like to know if it is possible to use S1 nuclease instead of P1 nuclease for gDNA digestion, prior to LC-ESI-MS/MS genome methylation determination?

Here is the base article:

Song, L., James, S. R., Kazim, L., & Karpf, A. R. (2005). Specific Method for the Determination of Genomic DNA Methylation by Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry. Analytical Chemistry, 77(2), 504-510. https://doi.org/10.1021/ac0489420

Thank you for your answers!

Adam Karpf

Yes, you can.

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