You can buy a kit for this procedure:

https://www.thermofisher.com/order/catalog/product/21277#/21277

Follow-up discussion

Dear Dr. Adam B Shapiro and Dr. Sebastian Schmitt,

Thank you very much for your comment. I also had a doubt about unspecifically bound proteins.

Thanks again,

Amal.

Hi Amal,

I have previously compared the result about GS-4B and Ni-NTA pull-down their corresponding tagged proteins and found that there are many non-specific proteins in the Ni-NTA group.

Dear Roger Davis,

Thank you for your valuable comment. I understand now, Ni-NTA is not a good choice for pull-down assay.

Thanks again.

Amal.

https://www.researchgate.net/post/Pulldown_assay_using_NiNTA_resins

Thanks,

Dr. Utsav Poudel.

Amal.