What is your suggested method and materials for producing UV-curable transparent coatings?

What are the easy methods for synthesizing UV curable resins? I need to synthesis UV curable coatings. MMA seems to have good features for transparent coatings, but the additives and synthesizing...

24 April 2020 3,256 7 View

What is the command for running comsol in the linux os?

I'm using comsol multiphysics on a cluster that operate linux os. i used this command to run and solve my mph file that named s4: comsol batch inputfile s4.mph outputfile s4solve.mph but the...

25 February 2020 5,691 1 View

How can one learn about the effects of an organic substance on the human body?

For example, we synthesized organic matter called A, at this point we want to know its effects on the human body. I wanted to know if there was a special device for this or were they using other...

16 December 2019 2,223 3 View

In immunofluorescence assay, do we need to wash away (with 1X PBS) blocking buffer (10% FBS-PBS) before adding the first antibody?

In immunofluorescence assay, do we need to wash away (with 1X PBS) blocking buffer (10% FBS-PBS) before adding the first antibody?

25 September 2019 6,591 3 View

How can I attach an amine group (-NH2) to a carboxyl group (-COOH)?

20 September 2019 5,256 5 View

I need a method for plating nano-silicon on a metal substrate?

Coating of metals with silicon

13 August 2019 1,318 3 View

Is it possible to simulate hysteresis effect for piezoelectric material in COMSOL or ansys?

I tried sinusoidal input voltage with the amplitude of 100 V but couldn't get any hysteresis curve

24 May 2019 5,973 4 View

Taqman probe real time PCR...Problem regarding constructing plasmid dna standard curve?

Hi guys......i am facing problems regarding developing standard curves for different copies of plasmid DNA using taqman probe real time PCR. i have successfully obtained standard curve for...

06 March 2018 2,284 3 View

Plasmid DNA copy number dilution?

Hello everybody..... the concentration of my plasmid DNA is 126ng. i want to do real-time PCR taqman probe detection......i want to do serial dilution that should start from 10e8 copy number....so...

21 February 2018 3,230 3 View

Looking for a learning tutorial for TSOL software?

Hello I am trying to find a learning tutorial for TSOL software. I would be thankful if you help me in this way Best Regards Mehran Dehghan

27 October 2017 8,799 3 View

No title

02 March 2021 3,060 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View