What public open software would you recommend for designing HRM primers?

What public open software would you recommend for designing HRM primers?

12 October 2020 3,925 4 View

ISH or immunostaining of GABA neurons?

Hi, Can I reliably label GABA neurons using ISH instead immuno-staining? It is extremely difficult to stain GABA neurons in VLPO and MnPO of hypothalamus by antibody and it seems that ISH is more...

21 September 2020 2,503 4 View

Are there any approved products that can prevent or treat COVID-19 in animals?

10 September 2020 8,643 19 View

Are my birds safe from COVID-19, and is my family safe around my birds?

07 September 2020 4,102 17 View

What tests can be used for a random number generator besides diehard?

What tests can be used for a random number generator besides diehard? Need find tests for my random number generator, based on human-computer interaction.

15 May 2020 7,167 7 View

What is the average depth explored in metal oxide samples through the following characterization techniques: XRD, XPS and Raman spectroscopy?

Obviously, the exact depth evaluated by each characterization technique mentioned (XRD, XPS and Raman spectroscopy) depends on the specific parameters adopted for each analysis. However, in...

15 May 2020 6,418 16 View

How can I add the journal name if your system does not find it in the list?

Hi, I have the published papers (with DOI) in the academic journals that have official ISSN etc. But I can not add and save the names of the journals in my records because your system does not...

26 April 2020 9,394 5 View

What are the storage conditions for phosphines for Staudinger ligation?

Hi all, We would like to purchase 5 mg of phosphine-modified fluorescent dye for site-specific labeling of proteins containing the unnatural aminoacid azide-phenylalanine. We will be labeling...

05 March 2020 3,007 3 View

Metallic materials (more specifically, nickel) present characteristic peaks in Raman spectroscopy analysis?

When evaluating an alumina sample using Raman spectroscopy, characteristic peaks were obtained for certain wavelengths. However, after depositing a thin nickel film via plasma on the surface of...

24 January 2020 8,954 3 View

Does anybody have PDF version of MOE manual?

I will highly appreciate if you can share one for program version 2014 or higher.

28 May 2019 5,615 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View