Hello. The question I wanted to pose to you is concerning in vivo assays. One of the possibilities of assays is a bronchoalveolar lavage (BAL). This technique entails que lavage with PBS (for example) of the airways, and collect that fluid, and further analyse it (proteins, enzymes and cells).
In your expertise, can we store the liquid from BAL? Like freezing, for example.
Or do we have to analyse right away?
Do you have any advice on how to tackle this?
Thank you all for your help in advance!
Hi Jorge,
We have 2 policy for this issue. If BAL analysis and cytology is about to done in hours, we send fresh fluid (BAL) to Lab. For those specimen, which maybe evaluated after longer period, we routinely add equal amount of 96% alcohol (50, 50) as cell fixator.
Thanks
Hello Jorge,
The BALF can be stored for testing later, but the storing way depends on what you wanna test. For example, if you wanna test IL-6 in BALF, you can centrifugate the BALF under the specific parameters (i.e., rotate speed, time, temperature) and store the supernatant at -80℃ directly right away. You can get the specific storing protocols from the published papers, protocol websites, the reagent companies, etc. Maybe, different papers will give the different protocols, just pick one that looks reliable and do the pre-testing. If it works, bingo! If not, try the other protocols.
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