Jorge F. Pontes

3 Questions 5 Answers 0 Followers

Questions related from Jorge F. Pontes

Is there any reference levels of IgE in healthy mice serum, like there are for other biochemical processes/components?

My question goes for reference levels of IgE in mice serum. I know that there are tables for the most common biochemical processes/compounds (red blood cells, white blood cells, AST, ALT, etc),...

07 January 2021 8,367 3 View

What is the most adequate (serum) dilution to use to determine IgE in mice serum via ELISA assay (commercial kit)?

We have a commercial kit from RayBioTech to determine IgE from mice serum. In accordance with the supplier's instructions, we have to use a dilution of 1:10000 of the serum. Is this dilution the...

07 January 2021 4,391 3 View

Is it possible to store the fluid from BAL, to analyse later (protein, enzymes and cells)? Or do we have to analyse straight after obtaining it?

Hello. The question I wanted to pose to you is concerning in vivo assays. One of the possibilities of assays is a bronchoalveolar lavage (BAL). This technique entails que lavage with PBS (for...

09 October 2020 6,070 3 View