I am currently working with Dendrolima pini and would like to store its eggs for a longer period of time >2 month. Does anyone know if you can freeze them or store them in liquid nitrogen?
Dear Jai Gosh,
Thank you very much for your answer! This is very helpful!
Kind Regards,
Lana Dobrindt
I stored Spodoptera Litura eggs at -80C. After 2 weeks I check they hatch successfully, which shows that we can store at -80 for longer period.
Hi Asim,
This is very imperssive, I did not know that this really works. Thank you very much for your answer!
Hi Asim,
I have a follow-up question considering the storage of Spodoptera eggs. Could you please tell me, how old those eggs werde before storage?
Hi Lana Dobrindt
I use freshly laid eggs. Please prefer to store fresh eggs. When females lays eggs then within few hours eggs incubation period starts depending upon temperature and humidity. it will be so good if you will use fresh eggs for storages. Because if you will use those eggs whos incubation period starts then cold storage damages their internal process by making them unable to hatch.
Have a good day
Hi Asim Munawar,
Thank you very much for the great answer. Did you put the eggs in the freezer directly or did you treat them somehow before freezing (like liquid nitrogen or something like that)?
Thank you, also a good day to you!
Hi Lana Dobrindt
I just collect fresh eggs from my rearing cages into cleaned petri dishes, wrapped them with Aluminum Foil and directly place them under -80.n
For You: Please check their hatching ability after different days intervals, by which you can determine for how many days you can store eggs for successful hatching.
Hi Asim Munawar,
Thank you very much for your answer and for the advice.
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Condensed Matter Physics
- Soft Matter
- Liquids