I have three target genes (hepcidin, MHC and IL), all of them are expressed from the same tissue (kidney). I will quantify their relative gene expression (qPCR) using beta actin as my internal control. Given that all these four genes (target genes plus internal control) have the same thermal profiles, can I run my internal control once and use its cp value to compute the relative expression of my target genes? I am thinking to do this in order to save reagents since they all came from the same tissue and same thermal profile.
Short answer: no.
Variability in the PCR reagents, fluorescence intensities of the probes and temperature uniformity of the thermocycler plate pretty much means that you have to run your positive controls on every plate.
You do not necessairly need to run multiple points of a standard curve, just a single one should suffice, so long as it is a sample from the original standard curve and has been stored appropriately (i.e. not gone through freeze/thaw).
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