Dear Muhammad,

The second part of your question you can easily look up in many NMR textbooks. Tim Claridges book would be one example. "High-Resolution NMR Techniques in Organic Chemistry"  and there are many more.

As for  the first part it is unlikely that you will be able to do this in a 2D NOESY experiment This would require an experiment with very high resolution. It would take a very long time to acquire. A possible solution would be a 1D selective NOESY or NOEDIFF This can be acquired with the resolution of a standrad 1D experiment. It requires selective excitation or saturation. You will have the best chance by running this at the highest field available. At 400 your signals will only 8 Hz apart. If you can get access to a 800 you now have 16 Hz to work with. Still very challenging.  The other possible solution would be a 13C edited NOESY. If the separation between the signals is larger in 13C than in 1H then running a  HSQC-NOESY might give your the answer.

Clemens

Well, it does depend on the strength of the magnetic field of your spectrometer. At 23.5 T (frequency of 1 GHz for 1H) you have 20 Hz per 0.02 ppm. Whether that's enough depends on the spectrum in question.

Regards,

Ludger Ernst

Thanks Clemens & Ludger.

It seems i have to do more reading in NMR to figure out all possibilities. 

Dear Muhammad,

what works if you have enough material is to observe the NOE between the 13C satellites of on line with the C12 center-line of the other compound. With a difference of typically 65 Hz (which is already 0.1 ppm at 600 MHz) there is some hope...

Hope it helps

Alfred